whey protein bands Search Results


image studio software  (LI-COR)
99
LI-COR image studio software
Image Studio Software, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blank dynabeads protein g  (Thermo Fisher)
97
Thermo Fisher blank dynabeads protein g
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Blank Dynabeads Protein G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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whey protein bands  (Bio-Rad)
99
Bio-Rad whey protein bands
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Whey Protein Bands, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantity one software  (Bio-Rad)
96
Bio-Rad quantity one software
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Quantity One Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sypro ruby  (Thermo Fisher)
90
Thermo Fisher sypro ruby
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Sypro Ruby, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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kda ladder band  (Bio-Rad)
97
Bio-Rad kda ladder band
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Kda Ladder Band, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti dhrs3  (Proteintech)
92
Proteintech anti dhrs3
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Anti Dhrs3, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl western blotting detection reagent  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc ecl western blotting detection reagent
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Ecl Western Blotting Detection Reagent, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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120a phenylthiohydantoin derivative analyser  (Thermo Fisher)
90
Thermo Fisher 120a phenylthiohydantoin derivative analyser
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
120a Phenylthiohydantoin Derivative Analyser, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gs365 scanning densitometer  (Bio-Rad)
90
Bio-Rad gs365 scanning densitometer
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Gs365 Scanning Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adjuvant complete h87 ra  (Difco)
90
Difco adjuvant complete h87 ra
INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from <t>Protein</t> <t>G</t> beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.
Adjuvant Complete H87 Ra, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adjuvant complete h87 ra - by Bioz Stars, 2026-03
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INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from Protein G beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.

Journal: Genetics

Article Title: A Family of Auxiliary Subunits of the TRP Cation Channel Encoded by the Complex inaF Locus

doi: 10.1534/genetics.120.303268

Figure Lengend Snippet: INAF-C associates with TRP, and contributes to the light response. (A) Expression level of TRP in P[SBP::trp] transgenic flies. Western blot showing the expression levels of TRP in head extracts prepared from w1118 and P[SBP::trp];trp343 flies. The blot was probed with rabbit anti-TRP and mouse anti-tubulin (Tub). Protein size markers (kDa) are indicated. (B) Representative ERG responses to orange light using the indicated flies; n ≥ 5 for each genotype. Time and mV scale bars are presented. (C) The four inaF mRNAs encode distinct proteins: INAF-A, INAF-B, INAF-C, and INAF-D. The colored rectangles indicate the protein-coding regions, the thick gray lines represent untranslated regions, and the thin black lines indicate introns. A DNA scale bar is provided. (D) Co-IPs to assess whether TRP associates with INAF-C::HA. Anti-HA (mouse) was used to perform IPs using head extracts prepared from w1118 and inaF-CHA, which harbors an HA tag knocked into the C terminus of INAF-C. The IPs were fractionated by SDS-PAGE, and Western blots probed with rabbit anti-HA (top; recognizes INAF-C::HA) or rabbit anti-TRP (bottom) primary antibodies and LI-COR anti-rabbit IRDye 800CW secondary antibodies. The positions of protein size markers (kDa) are shown to the left. The rabbit anti-HA also reacted with Protein G, which was released from Protein G beads upon elution. (E) Pulldown assays to determine whether INAF-C associates with TRP. Head extracts were prepared from inaF-CHA and inaF-CHA;P[SBP::trp];trp343 flies, the latter of which expresses the SBP::trp transgene in a trp343 null background. Head extracts were incubated with streptavidin beads, and the eluted proteins (streptavidin lanes) were fractionated by SDS-PAGE. The Western blots were probed with rabbit anti-TRP (top) and rabbit anti-HA (bottom) to identify TRP and INAF-C::HA, respectively. The input represents 4% of the extracts offered in the assays (D and E), and protein size markers (kDa) are shown to the left. (F) Wild-type inaF and inaF mutant alleles. Deletions are indicated by red brackets. (G–O) Representative ERG responses from the indicated genotypes (n ≥ 5 for each). A prolonged depolarizing afterpotential (PDA) is indicated in (G). Orange (O) and blue light (B) stimuli (5 sec/pulse) are indicated. Time and mV scales are indicated.

Article Snippet: The IP products common to the w 1118 and inaF-C HA samples (between 25 and 35 kDa; ) appeared to correspond to the protein G because we detected similar bands even when we eluted blank Dynabeads Protein G (Catalog# 10003D; Invitrogen) with 2× Laemmli sample buffer.

Techniques: Expressing, Transgenic Assay, Western Blot, SDS Page, Incubation, Mutagenesis